Experimentation capabilities
The iBiofoundry system is currently housed in BSL-1 workspace and integrated using Thermo MomentumTM scheduling software. The platform has been designed for accommodating a wide variety of applications, allowing flexibility for automated as well as manual standalone usage. The major instruments are listed below. Some of the established workflows on iBiofoundry include:
- PCR and PCR clean up
- DNA cloning (Gibson assembly, Golden Gate, PfAgo based cloning etc)
- Site-directed mutagenesis
- Solid phase extraction
- Microbial transformations and growth
- Protein expression
- Enzyme assays
Robotic liquid handlers
- Nanoliter-scale Labcyte Echo 650 acoustic liquid handler.
- Tecan Fluent 1080 with many on-deck modifications.
- Assisted by three more liquid handlers from iBioFAB.
Analytical instrumentation
- Sciex Echo-MS
- Lunatic plate reader
- Agilent Fragment Analyzer 5400
- QuantStudio QS7 Pro
- Tecan Spark Multimode Microplate Reader
- Singer PIXL Colony Picker
Peripheral instrumentation
- Nanoliter-scaBiotek Multiflo + (LHC2)
- Thermo ATC thermal cyclers 2x
- Thermo ALPS 5000 Sealer
- Thermo Multidrop Combi 2x
- KingFisher Presto 24 and 96 DW
- Bionex Xpeel
- 2x Teleshakes
- Scinomix Sci-Print MP2 plate labeler
- 4titude Sealer
- Bionex HiG3
